Validating high complexity lab test methods

Posted by / 10-Nov-2017 10:13

Second, everything we have learned to date on the risk relationship between cardiovascular disease and risk markers is predicated on the assumption that a risk maker of level X in a person on diet A is the same as it would be for a person on diet B.Since virtually all of the thousands of subjects who have made up the dozens of studies that form the basis for our understanding on this topic were consuming some variant of the “standard American diet” (i.e., high-carb), it is quite possible that what we know about risk stratification is that this population is not entirely fit for extrapolation to a population on a radically different diet (e.g., a very-low carbohydrate diet or a ketogenic diet).Before we get there, since this series has been longer and more detailed than any of us may have wanted, it is probably worth reviewing the summary points from the previous posts in this series (or you can just skip this and jump to the meat of this post). The question posed above did not ask how one could “prevent” or eliminate the risk cardiovascular disease, it asked how one could “delay” it. To appreciate this distinction, it’s worth reading this recent publication by Allan Sniderman and colleagues.

Sniderman and many others would argue (and I agree) that age is a strong predictor of risk has to do with exposure to apo B particles — LDL, Lp(a), and apo B-carrying remnants.The figure below shows the changes in serum triglycerides via 3 different ways of measuring them.Figure A shows the difference in 24-hour total levels (i.e., the area under the curve for serial measurements – hey, there’s our integral function again! Figure B shows late evening (post-prandial) differences.Figure C shows the overall change in fasting triglyceride level from baseline (where sugar intake was limited for 2 weeks and carbohydrate consumption consisted only of complex carbohydrates). The group that had all fructose and HFCS removed from their diet, despite still ingesting 55% of their total intake in the form of non-sugar carbohydrates, experienced a decline in total TG (Figure A, which represents the daily integral of plasma TG levels, or AUC).However, that same group experienced the greatest increase in fasting TG levels (Figure C).

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This was a randomized trial with 3 parallel arms (no cross-over).